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Also, bile acids metabolism has been described as involved in atherosclerosis development: it is probably related to bacterial bile salt hydrolyzation, which can stimulate enlargement of atherosclerotic plaque through cholesterol accumulation and foam cell formation [68]

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Once calibration had finished, the cell plate was inserted into the analyser, and OCR was determined at basal and mitochondrial stress conditions by triplicate measurement cycles of 0.5 min mixing, 0.5 min waiting and 2 min measuring, carried out at baseline and after each port injection

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After a 48 h observation period, blood was collected by retroorbital puncture and the mice were euthanized by neck dislocation